Purification and properties of rat brain pyruvate carboxylase.
نویسندگان
چکیده
Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.
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عنوان ژورنال:
- The Biochemical journal
دوره 145 1 شماره
صفحات -
تاریخ انتشار 1975